Functional analysis of Lsm protein under multiple stress conditions in the extreme haloarchaeon Haloferax mediterranei

The Sm, like-Sm, and Hfq proteins belonging to the Sm superfamily of are represented in all domains life. These involved several RNA metabolism pathways. functions bacterial eukaryotic have been described, but knowledge about vivo archaeal remains limited. This study aims improve understanding Lsm their role using haloarchaeon Haloferax mediterranei as a model microorganism. genome contains one lsm gene that overlaps with rpl37e gene. To determine expression genes co-transcription both, reverse transcription-polymerase chain reaction (RT-PCR) analyses were performed under different standard stress conditions. results suggest is constitutive. Co-transcription occurs at sub-optimal salt concentrations temperatures, depending on growth phase. halophilic protein two motifs, Sm1 Sm2, sequence encoding Sm2 motif also constitutes promoter investigate biological functions, deletion mutant mutant, where remained intact, generated characterised. Comparison parental strain HM26 conditions revealed differences. Finally, swarming assays complex defined media showed greater capacity mutants.